Bryan H. Bellaire, Ph.D.
Assistant Professor
Iowa State University
College of Veterinary Medicine
Department of Veterinary Microbiology
and Preventive Medicine
1136 Veterinary Medicine
Ames, IA 50011
Office: (515) 294-1006
Fax: (515) 294-8500
bbella@iastate.edu
Education
1995 B.S. in Microbiology, Northern Arizona University, Flagstaff, Arizona.
2001 Ph.D., Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, LA., August.
Professional and Research Experience
1994 DOE Science and Engineering Research Semester Fellow, Battelle Laboratories, Richland, WA. Performed environmental microbiology research under the direction of Todd Stevens, Ph.D.
1995 – 2001: Doctoral student, Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana. Research directed by R. Marty Roop II, Ph.D.
2001 – 2006: Post-doctoral position, Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana. Principal Investigator: Jim Cardelli, Ph.D.Funded in part by an NSRA award (F32-AI056965-01) to BHB
2001 – 2003: Adjunct Instructor-Course Director Introductory Statistics for the Behavioral Sciences. Psychology 216. Louisiana State University-Shreveport. Gary E. Jones, Ph.D. Department Chair.
2006 – present: Assistant Professor, Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University.
Extramural Funding
2002 NIH-NIAID Postdoctoral Fellowship – Brucella abortus Alters Trafficking in Human Monocytes. F32-AI056965-01; Funded 8/01/2003 – 7/31/2006.
Intramural Funding
8/1/2007-6/30/2009 Healthy Livestock Initiative. Novel Anti-Niche Approach against Brucella Infections in Cattle. Funded: 2 years – $40,000.
9/1/2007-8/31/2009 USDA Formula Funds. Cellular Interactions of Mycoplasma hyopneumoniae and Porcine Respiratory Epithelial Cells. Funded: 2 years – $38,000.
Publications
Bellaire, B.H., C.L. Baldwin, P.H. Elzer and R.M. Roop II. 1999. The siderophore 2,3-dihydroxybenzoic acid is not required for virulence of Brucella abortus in BALB/c mice. Infect. Immun. 67: 2615-2618.
Parent, M.A., Bellaire, B.H., Murphy, E.A., R.M. Roop II, P.H. Elzer and C.L. Baldwin. 2002. Brucella abortus siderophore 2,3-dihydroxybenzoic acid (DHBA) facilitates intracellular survival of the bacteria. Microb. Pathog. 32(5): 239-248.
Bellaire, B.H., P.H. Elzer, S.D. Hagius, J.V. Walker, N.J. Booth, M.D. Edmonds, C.L. Baldwin, R. Freeland and R.M. Roop II. 2003. Genetic organization and iron-Responsive regulation of the Brucella abortus 2,3-dihydroxybenzoic acid biosynthesis operon, a cluster of genes required for wild-type virulence in pregnant cattle. Infect Immun. 71(4): 1794-1803.
Bellaire, B.H., P.H. Elzer, C.L. Baldwin, and R.M. Roop II. 2003. Production of the siderophore 2,3-dihydroxybenzoic acid is required for wild-type growth of Brucella abortus in the presence of erythritol under low iron conditions in vitro. Infect Immun. 71(5): 2927-832.
RoopII, R.M., B.H. Bellaire, E. Anderson and J.T. Paulley. 2004. Iron metabolism in Brucella, p. 243-262. In I. Lopez-Goni and I. Moriyon (ed.), Brucella: Molecular and cellular biology. Horizon Scientific Press.
Roop II, R.M., B.H. Bellaire, M.W. Valderas, and J.A. Cardelli. 2004. Adaptation of the brucellae to their intracellular niche. Mol. Micro. 52(3): 621-630.
Valderas, M.W., R.B. Alcantara, J.B. Baumgartner, B.H. Bellaire, G.T. Robertson, W.L. Ng, J.M. Richardson, M.E. Winkler, and R.M. Roop II. 2005. Role of HdeA in acid resistance and virulence in Brucella abortus 2308. Vet. Micro. 20;107(3-4): 307-12.
Bellaire, B.H., R.M. Roop II, J.A. Cardelli. 2005. Opsonized virulent Brucella abortus replicate within non-acidic, endoplasmic reticulum negative, LAMP 1 positive phagosomes in human monocytes. Infect. Immun. 73(6): 3702-3713.
B.C. Reed, C. Cefalu, B.H. Bellaire, J.A. Cardelli, T. Louis, J. Salamon, M.A. Bloecher and R.C. Bunn. 2005. GLUT1CBP(TIP2/GIPC1) Interactions with GLUT1 and Myosin VI: Evidence Supporting an Adapter Function for GLUT1CBP Mol. Bol. Cell. 16(9): 4183-4201.
Roux, C.M., N.J. Booth, B.H. Bellaire, J.M. Gee, R.M. Roop II, M.E. Kovach, R.M. Tsolis, P.H. Elzer and D.G. Ennis. 2006. RecA and RadA proteins of Brucella abortus do not perform overlapping protective DNA repair functions following oxidative burst. J. Bacteriol. 188(14): 5187-5195.
Snider, J.L, B.H. Bellaire and J.A. Cardelli. Both CagA-Dependent and –Independent Signaling Pathways Contribute to Helicobacter pylori – Induced Motility of Gastric Carcinoma Cells. J. Cell Biol. Accepted: Feb 2007.
Ulery BD, Phanse Y, Sinha A, Wannemuehler MJ, Narasimhan B, Bellaire BH. 2009. Polymer chemistry influences monocytic uptake of polyanhydride nanospheres. Pharm Res. Mar;26(3):683-90.
Invited Lectures
2005 Brody School of Medicine, Department of Microbiology. East Carolina University.
2005 School of Veterinary Medicine, Department of Veterinary Science. Louisiana State University, Baton Rouge.
2005 College of Veterinary Medicine and Biomedical Sciences. Texas A & M University, College Station.
2005 Department of Microbiology, College of Veterinary Medicine, Oklahoma State University. Stillwater, OK.
2006 Department of Biological Sciences, Texas Tech University. Lubbock, TX.
2007 Department of Microbiology, Des Moines University School of Osteopathic Medicine. Des Moines, IA.
1994 Undergraduate Fellowship: Science and Engineering Research Semester Sponsored by the Department of Energy. January – May.
1997 Arnold Ravin – Murial Rogers Fellowship. 41st Annual Wind River Conference on Procaryotic Biology. June.
1998 The Donald E. Kahn Memorial Award for the Outstanding Graduate Student Presentation. 79th Annual Conference for Research Workers in Animal Diseases. November.
1998 Arnold Ravin – Murial Rogers Fellowship. 42nd Annual Wind River Conference on Procaryotic Biology. June.
1999 Arnold Ravin – Murial Rogers Fellowship. 43rd Annual Wind River Conference on Procaryotic Biology. June.
2000 Arnold Ravin – Muriel Rogers Fellowship. 44th Annual Wind River Conference on Procaryotic Biology. June.
2000 McCleskey Award for the Outstanding Graduate Student Presentation in
Bacteriology. South Central Branch American Society for Microbiology. November.
2001 Outstanding Postdoctoral Poster Presentation. Ray A. Barlow Scientific Symposium. January.
2002 Arnold Ravin – Murial Rogers Fellowship. 46th Annual Wind River Conference on Procaryotic Biology. June.
2002 Outstanding Postdoctoral Poster Presentation. Ray A. Barlow Scientific Symposium.
2003 Travel Award. 1st Annual Mid-Atlantic Microbial Pathogenesis Meeting, February 2-4. Wintergreen, Virginia.
2005 Outstanding Postdoctoral Presentation. Graduate Research Day. LSUHSC-Shreveport, May.
2006 Arnold Ravin – Murial Rogers Fellowship. 50th Annual Wind River Conference on Procaryotic Biology. June.
2009 Honorary Member of Phi Zeta Veterinary Honors Society
Professional Societies
1994 – 1995: Beta-Beta-Beta Biological Honor Society (Northern Arizona)
1994 – Present: American Society for Microbiology
1996 – 1999: Research Workers in Animal Diseases
1996 – 2006: South Central Branch of the American Society for Microbiology
1996 – Present: American Association for the Advancement of Science
2002 – Present: American Society for Cell Biology
2006 – Present: Midwest Branch of the American Society for Microbiology
2009 – Present: Honorary Member of Phi Zeta Veterinary Honors Society
Appointments and Institutional Service
1999-2000: Executive Council of the Student Body: Graduate Student Representative
1999-2000: Graduate Student Council: Dept. of Microbiology Student Representative
1999-2000: Graduate Advisory Council: Student Representative
2000-2006: LSUHSC BSL3 Laboratory Manager
2003-2006: Co-founder and President of LSUHSC Postdoctoral Organization
2003-2006: Scientific Advisory Board for LSUHSC Research Core Facility Microscopy Section
2004-2006: LSUHSC Institutional Biosafety Committee
2006-present: ISU VMPM-BSL3 Laboratory Director
Yersinia Project Consultant for BSL3 Select Agent Certification.
2007-present: ISU-CVM Biosafety Laboratory Consultant
2007-present: Ad hoc reviewer for Journal of Swine Health and Production.
5/15/2007 USDA Review Panelist. Animal Protection and Biosafety Section (44.0).
Teaching Experience
2001 – 2003: Course Organizer-Introductory Statistics for the Behavioral Sciences. PSY- 216. (LSU-S)
2002 – 2006: Lecturer – Methods in the Biomedical Sciences: IDSP-117 (LSUHSC)
2003 – 2006: Lecturer – Bacteriology and Molecular Pathogenesis: MICRO-291 (LSUHSC)
2006 – Present: Lecturer-Microbial Pathogenesis MICRO 553, VMPM 586 (ISU)
2006 – Present: Lecturer-Bacterial Pathogenesis: VMPM 528 (ISU)
2007 – Present: Lecturer-Innate Immunity in Bacterial Diseases: VMPM 625 (ISU)
2007 – Present: Course Co-Organizer VMPM 604 (ISU)
Selected Published Abstracts
Bellaire, B. H., P. H. Elzer, C. L. Baldwin, and R. M. Roop II. Contribution of the siderophore 2,3-dihydroxybenzoic acid to the pathogenesis of Brucella abortus in the BALB/c mouse model. Proc. 78th Annu. Conf. Res. Work. Anim. Dis. 1997; Abstr. 26, p. 17.
Bellaire, B. H., P. L. Schuetze, R. M. Roop II, C. L. Baldwin, and P. H. Elzer. Identification and characterization of a siderophore biosynthesis gene from Brucella abortus. 1997. Proc. 77th Annu. Conf. Res. Work. Anim. Dis., Abstr. 3, p. 6.
Bellaire, B. H., R. M. Roop II, C.L. Baldwin and P.H. Elzer. Contribution of the Brucella abortus 2,3-dihydroxybenzoic acid (DHBA) biosynthesis operon to virulence in ruminants. Proc. 79th Annu. Conf. Res. Work. Anim. Dis. 1998; Abstr. 4.
Bellaire, B.H., C.L. Baldwin, P.H. Elzer and R.M. Roop II. Genetic organization and Fur-independent regulation of the Brucella abortus 2,3-dihydroxybenzoic acid siderophore biosynthesis operon. 44th Annu. Meet. Wind River Conf. Proc. Biol. 2000. Session 6. p. 41.
Bellaire, B.H., C.L. Baldwin, P.H. Elzer and R.M. Roop II. The siderophore 2,3-dihydroxybenzoic acid contributes to the virulence of Brucella abortus in ruminants. 100th General Meeting of the American Society for Microbiology. 2000. Abstr.B-17, p.44.
Bellaire, B.H., R.M. Roop II, and J.A. Cardelli. Trafficking and residence of Brucella abortus inside human monocytes. 2002. 42nd Annual Meeting for the American Society for Cell Biology. Abstr. B-697.
Bellaire, B.H., R.M. Roop II, and J.A. Cardelli. Brucella abortus controls phagosome membrane trafficking events throughout infection in human monocytes. 2003. 43nd Annual Meeting for the American Society for Cell Biology. Abstr. B-523.
Bellaire, B.H., A. Rupper, R.M. Roop II, and J.A. Cardelli. Interferon Gamma Induced Changes in Phagosome Maturation Inhibits the Intracellular Replication of Brucella spp. in Human Monocytes. 2005. 105th General Meeting of the American Society for Microbiology. Abstr. B-287.
Bellaire, B.H., A. Rupper, R.M. Roop II, and J.A. Cardelli. Interferon-γ Induced Changes in Phagosome Maturation Inhibits the Intracellular Replication of Brucella spp. in Human Monocytes. 2006. Virulence Mechanisms of Bacterial Pathogens International Symposium. Iowa State University.
B Ulery, J Wilson, SA Sarkar, M Torres, S Lopac, A Glowacki, B Bellaire, MJ Wannemuehler & B Narasimhan. In Vitro Evaluation of Polyanhydride Nanosphere Interactions with Human and Murine Antigen Presenting Cells. 2008. 6th Annual American Society for Microbiology Biodefense and Emerging Diseases Research Meeting.
Selected Presentations
Bellaire, B.H., P.L. Schuetze, R.M. Roop II , C.L. Baldwin and P.H. Elzer. Siderophore production contributes to the virulence of Brucella abortus in pregnant ruminants. South Central Branch Meeting of the American Society for Microbiology. 1996.
Bellaire, B.H., P.L. Schuetze, R.M. Roop II, C.L. Baldwin and P.H. Elzer. Characterization of a siderophore deficient mutant of Brucella abortus. 50th Annual Meeting of the Brucellosis Research Conference. 1997.
Bellaire, B.H., C.L. Baldwin, P.H. Elzer and R.M. Roop II. Contribution of the siderophore 2,3-dihydroxybenzoic acid to the virulence of Brucella abortus in ruminants. 51st Annual Meeting of the Brucellosis Research Conference. 1998.
Parent, M., B.H. Bellaire, J. Sathiyaseelan, R.M. Roop II and C. L. Baldwin. Growth and survival of an entC mutant of Brucella abortus strain 2308 in interferon-gamma knock-out mice and macrophages.52nd Annual Brucellosis Research Conference. 1999.
Bellaire, B. H., C. L. Baldwin, P. H. Elzer, and R. M. Roop II. Genetic organization and regulation of the Brucella abortus DHBA biosynthesis operon.52nd Annual Brucellosis Research Conference. 1999.
Bellaire, B. H., C. L. Baldwin, P. H. Elzer, and R. M. Roop II. 2,3-Dihydroxybenzoic acid production contributes to the virulence of Brucella abortus in ruminants. 52nd Annual Brucellosis Research Conference. 1999.
Bellaire, B. H., C. L. Baldwin, P. H. Elzer, and R. M. Roop II. Siderophore production contributes to the virulence of Brucella abortus in pregnant ruminants. South Central Branch American Society for Microbiology. 1999. Abstract B2-1, p. 32.
Bellaire, B.H., R.M. Roop II, J. Cardelli. Trafficking and residence of Brucella abortus inside human monocytes. 1st Annual Mid-Atlantic Microbial Pathogenesis Meeting. 2003.
Bellaire, B.H.,R.M. Roop II, J. Cardelli. Intracellular residence of Brucella abortus within interferon-gamma activated monocytes. 14th Annual Midwest Microbial Pathogenesis Meeting. 2007.